Using real-time polymerase cuain reaction (PCR), gene sequences can be amplified to the extent that species and strain of as little as two cells can be identified. This is a technique that is used in other laboratories; however, applications for molecular fingerprinting of cells related to weapons of mass destruction (WMD) are desired by Department of Homeland Security (DHS) and possibly by other sponsors.
Real-time PCR with fluorescence or electrophoresis detection has been used to generate a database to be used for identification. Emphasis has been on organisms such as Brucella spp. because it is a biocontaminant of interest. Research is currently conducted in a Biosafety Level 2+ facility. Microarray sensor instrumentation was recently procured, which should augment capability.
- Contacts:
- Gary Groenewold, (208) 526-2803, Send E-mail